Award details

Biotechnology Resources for Arable Crop Transformation (BRACT)

Reference	BBS/E/J/0000A159
Principal Investigator / Supervisor	Professor John Snape
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	295,626
Status	Completed
Type	Institute Project
Start date	01/06/2003
End date	28/02/2007
Duration	45 months

Abstract

The main project objective is to provide a highly efficient, robust transformation capability for the main UK arable crops while facilitating further improvements in transformation technology and delivery to the UK research community. The project will provide access to up to date transformation protocols for the main UK crops, wheat, barley and oilseed rape as well as some secondary crops. The project will also provide a new set of easy to use transformation vectors. Following extensive consultation with the UK research community, the transformation facility will be tailored to meet demand but will include training facilities, hotel facilities in transformation laboratories where individuals can carry out their own experiments with assistance as required, opportunities for collaborative projects and a full transformation service that will introduce genes of interest and deliver transgenic plants or seed. The project also has a number of scientific objectives including providing improved Agrobacterium-mediated transformation systems for oilseed rape, developing efficient Agrobacterium-mediated transformation for winter barley varieties and developing an efficient and reproducible Agrobacterium-mediated transformation system for wheat. In addition methodology for producing `clean-gene¿ transformants, containing only the gene of interest and no marker genes will be developed for the main UK crops. Studies on methods for the stabilisation of transgene expression will also form part of the project. A project web-site, www.bract.org, will contain up to date information on the project together with details of the transformation protocols and the new transformation vectors.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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