Award details

Cloning and analysis of key legume symbiotic genes with the use of synteny between pea (Pisum sativum L.) & Lotus japonicus (Regel.) K. Larsen genomes

Reference	BBS/E/J/0000A092
Principal Investigator / Supervisor	Professor Thomas Ellis
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	4,090
Status	Completed
Type	Institute Project
Start date	01/07/2002
End date	30/06/2005
Duration	36 months

Abstract

1.To characterise the pea gene PsNin homologous to LjNin. 2.To study the pattern of expression of the PsNin gene in indeterminate nodules of pea. 3.To carry out the genetic transformation of L. japonicus nin mutants and pea sym35 mutants with functional copies of both LjNin and PsNin to obtain novel models for studying the expression of this type of transcriptional activator and to have one more proof about homology between PsSym35 and LjNin. 4.To perform fine mapping of the segment of pea genetic map carrying PsSym31 with the use of molecular markers to analyse synteny between L. japonicus and pea genomes in the region of genetic map presumably carrying LjSym31. 5.To identify mutants presumably carrying the mutation in L. japonicus gene homologous to PsSym31 by screening the collection of L. japonicus non-fixing mutants for those having the same features as pea mutant in PsSym31 gene. 6.To map L. japonicus non-fixing mutations in the gene(s) presumably homologous to PsSym31 gene with the use of molecular markers to create conditions for positional cloning of the gene(s).

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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