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Characterisation of the TRH1 gene which encodes a putative potassium transport protein in Arabidopsis

Reference	BBS/E/J/0000A045
Principal Investigator / Supervisor	Professor Liam Dolan
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	48,289
Status	Completed
Type	Institute Project
Start date	30/10/2001
End date	29/10/2003
Duration	24 months

Abstract

The TRH1 mutant produces short root hairs implying a role for the TRH1 gene in root hair growth. Cloning of TRH1 revealed that it encodes a putative K-carrier which transports K+ across membranes at low efficiency. A similar K-transporter ATK1 has been previously described however it is not known across which membranes the two proteins transport K+. It is proposed that either they work at different concentrations of external K or that they transport across different membranes (e.g. PM or tonoplast membrane) at similar K+ concentrations. This project is structured to address this question and to provide training in the techniques involved. This project involves the localisation of the activity of the TRH1 gene using a variety of molecular and microscopical techniques. These include in situ hybridisations, the construction and localisation of a TRH1::GFP fusion protein and the localisation at the TEM level using immunoglod labelling. As well as localising gene expression to a particular membrane the level of expression of both the TRH1 and the ATK1 will be examined at different external K concentrations. These experiments will give an insight into TRH1 function and its interaction with ATK1.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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