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Molecular determination of the epigenetic basis of vernalization
Reference
BBS/E/J/0000A041
Principal Investigator / Supervisor
Professor Dame Caroline Dean
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
John Innes Centre Department
Funding type
Research
Value (£)
85,783
Status
Completed
Type
Institute Project
Start date
04/03/2002
End date
03/09/2005
Duration
42 months
Abstract
Recent data suggest that vernalization is controlled by an epigenetic mechanism involving Polycomb Group (PcG) genes. One of the Arabidopsis genes (VRN2) that mediates vernalization is homologous to Drosophila Su(z)12, a Polycomb Group gene. In addition, VRN1 and VRN2 are required for the maintenance of repression of RNA levels of a target gene, FLC rather than in the initial repression. To pursue this paradigm, we will purify multi-protein complexes involving VRN1 and VRN2 using a double affinity tag and identify the protein components using tryptic fingerprinting and MALDI-ToF. We will also determine the preferred VRN1 DNA-binding sites using SELEX and analyse whether VRN1 directly binds to FLC using gel-shift assays.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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