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Integration of antagonistic signals: role of protein-protein and protein-ligand interactions in nitrogen and carbon regulation

Reference	BBS/E/J/0000A033
Principal Investigator / Supervisor	Professor Raymond Alan Dixon
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	46,487
Status	Completed
Type	Institute Project
Start date	01/05/2002
End date	01/05/2005
Duration	36 months

Abstract

We have recently demonstrated that the signal transduction protein PII interacts with the NIFL-NIFA system to mediate nitrogen regulation. We propose to characterise this protein-protein interaction and study how the signal for nitrogen regulation is opposed in response to carbon status by the metabolite 2-oxoglutarate. We plan to use a combination of surface plasmon resonance (SPR), chemical cross-linking and mutagenesis to delineate the interaction surfaces in the PII-NIFL binary complex and determine how the formation and stability of this complex is influenced by uridylylation of the PII protein and ligand binding to both protein components.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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