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Electron nuclear double resonance studies (ENDOR) of enzymes

Reference	BBS/E/J/00004043
Principal Investigator / Supervisor	Professor David John Lowe
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	339,141
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/2001
Duration	48 months

Abstract

Structural and electronic characterisation of ligands to paramagnetic metal sites in enzymes ENDOR (Electron Nuclear Double Resonance) is a spectroscopic technique for studying electronic and geometric structure at distances of 0.5 to 1.0 nm from paramagnetic centres. Such centres occur in chemical systems, and in proteins containing transition metals and organic free radicals like flavin semiquinone. The knowledge obtained is complementary to other techniques and can give single-crystal quality data using frozen solutions so that mixtures of molecules and unstable intermediates can be examined. It is possible to study complexes with substrates/inhibitors and protein/protein interactions. JIC has become the principal laboratory in the UK and is recognised as a world centre of excellence for the use of this sophisticated procedure. ENDOR is used, in conjunction with other methods, on a wide variety of interdisciplinary projects including those associated with ROs 4026, 4032, 4035, 4037, 4048, 4049, 4050, 4051 and 4056.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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