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Gene regulation in transgenic wheat
Reference
BBS/E/J/00003527
Principal Investigator / Supervisor
Professor John Snape
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
John Innes Centre Department
Funding type
Research
Value (£)
10,233
Status
Completed
Type
Institute Project
Start date
01/01/1999
End date
31/03/2001
Duration
27 months
Abstract
The main aims of this project are to add SAR, actin terminator and lox sequence to the expression cassettes; Evaluate affect of adding introns to the luciferase ORF; Transformation of wheat using constructs pAL250 and pAL253; Characterization of the TO parent; Determination of luciferase transgene copy number and quantification of its expression in single seed derived populations; Determine if genetic background significantly alter expression; Mapping transgene loci using the chinese spring monosomics; In situ hybridization to localize position of transgene on chromosome; Recovery of transformed plants from transformed callus.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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