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Mapping T-DNA integrations carrying Ds elements on the Arabidopsis genome

Reference	BBS/E/J/00003503
Principal Investigator / Supervisor	Professor Dame Caroline Dean
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	16,711
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	01/04/1999
Duration	24 months

Abstract

We aim to map 100 T-DNA integrations carrying Ds elements in order to greatly improve the efficiency of targeted insertional mutagenesis in Arabidopsis. New sAc and Ds elements have been constructed that allow transposition events to be selected in the greenhouse. Landsberg errecta transformants with single copy T-DNA integrations carrying the Ds elements have been generated and plant DNA flanking the insertion isolated. The flanking DNA is hybridised to arrayed YAC clones that form the physical map covering all five chromosomes. Targeted tagging experiments will be undertaken to provide information on the number of families that need to be screened to obtain a particular mutation.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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