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Reproducible Agrobacterium-mediated transformation systems for wheat and barley

Reference	BBS/E/J/00003349
Principal Investigator / Supervisor	Professor John Snape
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	23,228
Status	Completed
Type	Institute Project
Start date	01/10/2000
End date	30/09/2001
Duration	12 months

Abstract

This MAFF funded project was set up to develop efficient protocols to be able to genetically engineer wheat and barley via Argobacterium. This would have advantages over biolistic approaches in terms of low copy number and stable insertions, giving better gene expression and stability. New constructs were developed and different parameters tested to optimize an approach. Both barley and wheat were successfully transformed using the protocols developed, but frequencies were lower than biolistic approaches. Further work will be required to further optimize the procedures.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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