Award details

Development of wheat allele specific oligos (ASOs) from microsatellite markers and their use in developing multi-locus, multi-allele genotyping assays

ReferenceBBS/E/J/00003228
Principal Investigator / Supervisor Dr Robert Koebner
Co-Investigators /
Co-Supervisors
Institution John Innes Centre
DepartmentJohn Innes Centre Department
Funding typeResearch
Value (£) 122,070
StatusCompleted
TypeInstitute Project
Start date 01/04/2001
End date 31/03/2004
Duration36 months

Abstract

Wheat is the most important cereal grown in the UK. Unfortunately, in terms of molecular breeding, it is also the most difficult. Current wheat molecular markers such as RFLPs, AFLPs and SSRs, are limited to gel-based technologies. In this proposal we intend to utilise the highly polymorphic flanking regions of existing microsatellite markers to develop allele specific oligonucleotides. In turn these ASOs will be exploited in the development of a DNA genotyping chip and MALDI-TOF analysis to characterise wheat genotypes or varieties. The sequences developed by this programme will be of considerable academic and commercial interest.

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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