Award details

Study of biology of fungal disease and the genetic engineering of rice for enchanced disease resistance

Reference	BBS/E/J/00001708
Principal Investigator / Supervisor	Professor Chris Lamb
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	4,571
Status	Completed
Type	Institute Project
Start date	01/10/1999
End date	31/12/2006
Duration	87 months

Abstract

This project centres on increasing our understanding of the biology of fungal disease and engineering of rice for enhanced disease resistance. We have produced transgenic plants expressing the antifungal protein Glucose Oxidase (GOX). In addition to the PAL::GOX , we shall evaluate the use of the 35S::RCHIO chitinase and 35S::AGLU1 glucanase double constructs. These constructs have shown some promise against Rhizoctonia. Likewise, for UBG: HIvIGR plants which show enhanced isoprenoid biosynthesis and protection against bacterial blight, will be tested for blast and sheath blight. Combinations of these genes in the same background will be achieved by crossing or co-transformation. Constructs of a chimeric receptor with the N-terminal putative extracellular domain of the BIN1 steroid signalling LRR-receptor kinase fused to the kinase catalytic domain of the Xa21 LRR kinase disease resistance gene have been recently. Preliminary work in cell cultures shows steroid inducible hypersensitive cell death and PR-gene induction. Transgenics are currently being regenerated. We shall use these transgenics for further evaluation. We shall attempt to express in rice, signal transduction genes involved in SAR. The best characterized to-date are CDR1 and DIR1 both of which confer quite broad protection when over-expressed in Arabidopsis. This work shall involve making appropriate constructs for expression in rice and then disease evaluation. In addition, it would also involve the isolation and manipulation of the rice homologue.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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