Award details

Comparative genome analysis and mapping in conifers

Reference	BBS/E/J/00001312
Principal Investigator / Supervisor	Professor J Heslop-Harrison
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	1,937
Status	Completed
Type	Institute Project
Start date	01/08/1997
End date	31/07/2000
Duration	36 months

Abstract

The main objective of the proposed research is to improve our basic understanding of genome organisation in conifers. This will be achieved by developing and utilising a series of innovataive, generic enabling technologies that will be used to characterise conifer genomes at various tiers of organisational complexity. Emphasis will be given to the following objectives: a) an examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family; b) determine level of synteny or conservation of gene order between species within the Pinaceae family. Integrating genetic maps and models. Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. Conifers are diploids which exhibit conservation of chromosome number (2n=2x=24). Initial studies with isozymes (1) indicate that pines exhibit conservation of gene order and this hypothesis will be examined further by the use of transcript based linkage maps.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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