Award details

A concerted approach to global analysis of gene function in Streptomyces coelicolor

Reference	BBS/E/J/00001239
Principal Investigator / Supervisor	Professor Keith Chater
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	21,004
Status	Completed
Type	Institute Project
Start date	01/10/2000
End date	30/09/2003
Duration	36 months

Abstract

This is a supplementary award to grant 208/IGF12436 in which resources will be provided for systematic analysis of gene function in S. coelicolor. Transposon libraries containing random insertions will be phenotypically characterised and the disrupted genes identified. PCR based procedures will be developed for large- scale targeted mutagenesis by gene replacement with linear cassettes. Phage based vectors for insertional mutagenesis will be developed and exploited to insert regulated promoters to control essential genes, and to transduce insertions to form multiple mutants. Detailed functional analysis will focus on putative regulatory genes. All resources and methods will be made widely available, together with access to a co- ordinated database of all mutants, linked to existing S. coelicolor databases. (Joint with grants 58/IGF12431 and 208/IGF12432.)

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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