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Concerted approach to global analysis of gene function

Reference	BBS/E/J/00001236
Principal Investigator / Supervisor	Professor Keith Chater
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	297,238
Status	Completed
Type	Institute Project
Start date	03/04/2000
End date	03/04/2003
Duration	36 months

Abstract

We will provide resources for systematic analysis of gene function in S. coelicolor. Transposon libraries containing random insertions will be phenotypically characterised and the disrupted genes identified. Linear cassettes generated by PCR will be used for targeted gene replacement. Phage-based vectors for insertional mutagenesis will be developed and exploited to insert regulated promoters to control essential genes, and to transduce insertions to form multiple mutants. Detailed functional analysis will focus on putative regulatory genes. All resources and methods will be made widely available, together with access to a co-ordinated database of all mutants, linked to existing S. coelicolor databases.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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