Award details

Rational process innovation and design for Streptomyces

Reference	BBS/E/J/00001219
Principal Investigator / Supervisor	Professor Mervyn Bibb
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	117,380
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/10/1999
Duration	31 months

Abstract

Streptomycetes, or more generally actinomycetes, are versatile and potentially high-yielding producers of proteins and secondary metabolises. Particularly the latter compounds have attracted industrial interest because many secondary metabolises display important pharmacological properties like antibiotic, antiviral, and anti-cancer activity, or have an agricultural application as (animal) feed conversion enhancer or herbicide. A large number of actinomycetes are currently being used as production microorganisms in large scale fermentation. However the development of actinomycete production strains and fermentation processes still is highly empirical and therefore unpredictable with respect to both speed and chance of success. The objective of this project is to integrate fermentation knowledge, metabolic flux analysis, and genetics of substrate utilization and regulatory systems to develop rational strain and process development procedures applicable to a variety of actinomycete strains. This will be achieved by emphasizing general properties of secondary metabolise production: except for the overexpression of secondary metabolise production by deregulation of biosynthetic pathway genes, the proposed project will only address basic genetic, biochemical, physiological, and fermentation properties that are conserved within the actinomycete family. This focus will ensure that the results will be applicable to strain and process development for a wide variety of actinomycetes. The actinomycete strain to be studied is Streptomyces lividans. The basic strain will resemble an actual production strain with major bottlenecks in the metabolise specific biosynthetic pathway already relieved.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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