Award details

Programming the commitment of aerial hyphae to sporulation in Streptomyces coelicolor

Reference	BBS/E/J/00001207
Principal Investigator / Supervisor	Professor Keith Chater
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	15,151
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	13/12/1998
Duration	20 months

Abstract

Streptomyces species produce spores on aerial hyphae. Understanding this process is important from two points of view: the preparation of Streptomyces inocula for industrial fermentations is more reliable when reproducible sporulation can be obtained; and such knowledge is relevant to general development biology, since the first sign of sporulation of aerial hyphae is the coupled cessation of extension growth and multiple cytokinesis in the syncytial hyphal tip. The aim of this project is to elucidate the physiological and genetic mechanisms of this reprogramming by the study of five genes whose activity is essential for normal sporulation septation. The sequencing of these (already cloned) genes will be completed, whiH mutants will be constructed, and the transcription patterns of all five genes in the wild-type and whiH mutants will be established. The phenotypes resulting from ectopic expression of the genes will be evaluated. The genes will be over- expressed in E. coli and the proteins purified and used to raise antibodies. The antibodies will be used to study the occurrence of the natural protein in differentiating Streptomyces colonies. Studies of DNA: protein interaction involving the five proteins will be carried out as appropriate.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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