Award details

Clean technology through the use of engineering polyketides

Reference	BBS/E/J/00001204
Principal Investigator / Supervisor	Professor Sir David Hopwood
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	5,791
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	30/09/1998
Duration	18 months

Abstract

A huge range of antibiotics and other bioactive compounds are made by the polyketide route, in which increments (often of two carbons) are sequentially added to a growing carbon chain, with modifications being made at each step. The application of molecular genetics to the study of one such compound, the benzoisochromanequinone (BIQ), actinorhodin, in the model organism Streptomyces coelicolor A3(2), has provided the basis for a multidisciplinary analysis of the programming of molecular specificity during polyketide biosynthesis. In this project, techniques, materials and collaborations established during earlier phases of the work will be exploited in an investigation of the formation of the BIQ heterocyclic pyran ring, and the establishment of the stereochemistry of its two chiral centres. Four BIQs will be studied from three pathways: two pathways give products with unique and different chiralities, and the third gives a mixture of both kinds of chirality. Potentially relevant genes will be identified and inactivated, and the resulting polyketide bioproducts will be characterised. Based on this information, isotopically labelled intermediates will be synthesised for use in feeding experiments to confirm details of the biosynthetic pathways.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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