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An examination of the pleiotropic effects that may result from multigene insertions and their outcrossing in tomato hybrids

Reference	BBS/E/J/00001137
Principal Investigator / Supervisor	Prof. Phillip Mullineaux
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	18,366
Status	Completed
Type	Institute Project
Start date	01/10/2000
End date	31/03/2004
Duration	42 months

Abstract

The project addresses the pleiotropic effects of transforming plants with large amounts of DNA, and the effects on genetic stability that crossing and selection may have on such large insertions. The intention here is to use the processor tomato FM6203 as a model system in which the main focus of the analytical screens will be the red ripe tomato fruit. The transgenes are from diverse species and include four enzymes from the glutathione metabolism pathway isolated from E.coli, and pea. To facilitate transformation there is an antibiotic selection gene from E. coli (kanamycin resistance) and a reporter gene, firefly luciferase. A second line has been prepared containing three enzymes from the ascorbate pathway, and contains a large contiguous area of foreign DNA in the region of 12.5Kb, and similar selection and reporter genes. Since the genes in the two transformants are collected together from diverse sources, and the site of genomic insertion is random, they may not behave as a predicable genetic locus during crossing to either another transgenic, or to a wildtype line. Protein and metabolite profiling using MALDI-TOF MS, and proton NMR will examine the pleiotropic effects, ie changes that are unpredicted¿.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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