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The roles of plastidial starch modifying enzymes in determining starch quantity and quality in plants

Reference	BBS/E/J/00001136
Principal Investigator / Supervisor	Professor Alison Smith
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	75,298
Status	Completed
Type	Institute Project
Start date	21/04/1999
End date	21/04/2002
Duration	36 months

Abstract

Starch-modifying enzymes are present in plastids during starch synthesis as well as during breakdown. They may thus play a key role in the determination of starch quality and quantity in plants. However, the precise functions of each of these enzymes during starch synthesis and during breakdown, are unknown. Our aim is to determine the function of each enzyme by studying starch structure and metabolism in Arabidopsis mutants lacking specific plastidial isoforms of debranching and disproportionating enzymes, phosphorylase, amylase and a-glucosidase. We have already identified three mutants lacking debranching enzyme, endoamylase and disproportionating enzyme, and will isolate others using a revere-genetics approach. Our studies will clarify unresolved issues such as the role of debranching enzymes in starch synthesis, and the impact of turnover on starch yield.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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