Award details

Development of marker-free ('Clean gene') transgenic crop plants using Agrobacterium-based clean gene technology

Reference	BBS/E/J/00001131
Principal Investigator / Supervisor	Prof. Phillip Mullineaux
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	56,262
Status	Completed
Type	Institute Project
Start date	01/10/1998
End date	01/10/2001
Duration	36 months

Abstract

The clean gene technology to be developed in this project uses existing Agrobacterium-mediated procedures and employs a newly developed highly flexible binary Ti vector system which has been funded by the BBSRC. This approach relies on the co- transformation into plants of two T-DNAs, only one of which contains the selectable marker gene. In a proportion of ransformants, the integration of the T-DNAs into separate loci will occur. Subsequently, a self-crossing of the transformant will lead to segregation of the marker T-DNA from aT-DNA carrying a test gene. The project will determine the best parameters for achieving the maximum frequency of segregation of the T-DNAs, provide a flexible plasmid system to encourage use, techniques to eliminate primary transformants with linked T-DNAs prior to transfer to containment glasshouse and a demonstration of its use in two UK crops.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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