Award details

Importance of malto-oligosaccharides in the synthesis of starch

Reference	BBS/E/J/00001116
Principal Investigator / Supervisor	Professor Alison Smith
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	2,084
Status	Completed
Type	Institute Project
Start date	03/11/1997
End date	02/11/2000
Duration	36 months

Abstract

In this project we will discover the extent and nature of the contribution of an isoform of starch synthase, granule-bound starch synthase I (GBSSI), to amylopectin synthesis in starch granules. The major product of GBSSI is amylose. This is synthesised inside the granule matrix by addition of glucose from ADPglucose to small malto- oligosaccharides. GBSSI can also elongate a specific class of long chains in the amylopectin matrix. These chains may be very important in determining the properties of the granule. We hypothesise that the extent to which GBSSI synthesises amylopectin chains rather than amylose may be influenced by the availability of malto- oligosaccharides as primers for amylose synthesis. To test this, we will attempt to manipulate malto- oligosaccharide levels in planta by creating transgenic plants which express enzymes of malto- oligosaccharide degradation in the amyloplast. We will also develop techniques to determine the precise locations of GBSSI and its products within the granule matrix.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search