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Transgene structure and function and stability in crop species important for UK agriculture

Reference	BBS/E/J/00001109
Principal Investigator / Supervisor	Dr Paul Christou
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	56,485
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	14/03/1999
Duration	23 months

Abstract

Transgene structure, function and stability in crop species important for UK Agriculture: The aim of the project is to develop an understanding of underpinning factors that influence transgene stability and expression in wheat. A large number of transgenic wheat plants were created by particle bombardment using marker genes and genes of agronomic interest. This population of plants is being used to analyse and define molecular, genetic and phenotypic events resulting from transformation using either cointegrate vectors or co- transformation. Comparisons with the molecular composition and expression of transgenes in rice plants also generated through bombardment illustrate similarities but more importantly some differences in terms of behaviour and stability of transgenes in the two systems, with wheat showing a bias towards higher levels of transgene expression in multicopy populations. In order to study the influence of position effect on levels and stability of gene expression we use fluorescence in situ hybridisation to localise transgenes on specific chromosome regions. This technique (which has been made available to us through a collaborative effort with Dr. Peter Shaw) in combination with detailed molecular analysis allows to make definitive statements on the structure, organisation and localisation of transgenes as a prerequisite for establishing a relationship between these factors and transgene stability in transgenic crop plants.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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