Award details

Genome structure, genetic mapping and interspecific gene transfer

Reference	BBS/E/J/00000456
Principal Investigator / Supervisor	Dr Martin Trick
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	53,114
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	31/03/1998
Duration	12 months

Abstract

Brassica napus is an amphidiploid species formed from the hybridisation of B. oleracea and B. rapa. 'Natural' B. napus lines possess a system which prevents recombination between homoeologous chromosomes. Synthetic B. napus, formed from new hybridisations between B. oleracea and B. rapa, lacks this stabilising mechanism allowing the transfer interesting genes between the B. oleracea and B. rapa genomes. The regions of homoeology between B. oleracea and B. rapa are being mapped and the frequency of homoeologous recombination estimated in a range of synthetic lines. The gene(s) which determine the stringency of chromosome pairing will be mapped, manipulated in breeding programmes and cloned. The investigation of genome collinearity and interspecific gene transfer in Brassica will be extended to include B. juncea which has a number of novel genes including some which promote drought tolerance and aphid resistance. 11 S-alleles have been introduced into B. napus from B. oleracea by interspecific hybridisation and embryo- rescue. These S-alleles will allow the development of self- incompatible (SI) oilseed rape. Oilseed rape possesses a suppressor of self-incompatibility, which is being mapped, and this suppressor will be replaced with a functional allele from the ancestral diploid species to improve the SI phenotype. The SI lines of oilseed rape will be used to produce F1 seed for future genetic analyses of heterosis. The collinearity between the genomes of Brassica and Arabidopsis is being investigated. This will allow researchers to utilise extensive Arabidopsis 'contigs' to facilitate chromosome walking and gene cloning in Brassica. It should also allow researchers to use Arabidopsis clones to identify potential Brassica RFLP markers localised in defined regions of the Brassica genome.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search