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Plant signal perception and signal transduction

Reference	BBS/E/J/00000131
Principal Investigator / Supervisor	Dr Bjorn Drobak
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	948,861
Status	Completed
Type	Institute Project
Start date	01/04/1997
End date	28/04/2006
Duration	108 months

Abstract

One of the main interests of my group is the role of Ca2+ and the phosphoinositide system in plant cell signalling. We have developed a wide range of methods for the analysis of signal-induced phospholipid turnover (in vivo and in vitro) and the production of lipid-derived second messenger molecules. We have discovered several types of plant GTP-binding proteins and are currently characterising their physiological roles. We have experience with a wide range of plant tissues as well as with mammalian systems. A more recent interest is the subcellular localisation (and translocation) of signalling enzymes and other components of signalling pathways. Techniques are available for the preparation of various subcellular fractions which include: whole-cell extracts, vacuolated protoplasts, cytosol, microsomal membranes, vacuolar membranes, plasma membranes, cytoskeletons and nuclei. Cellular Ca2+-fluxes are being investigated using both radiochemical methods and Ca2+-sensitive mini- electrodes. Recently we have acquired a UV-confocal laser scanning microscope and have initiated several new projects aimed at studying Ca2+-fluxes and other signalling events at the single-cell level.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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