Award details

Modification of the infectious bronchitis virus spike protein for growth in Vero cells; potential for vaccine growth and production in cell culture

Reference	BBS/E/I/00001524
Principal Investigator / Supervisor	Professor Paul Britton
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	349,330
Status	Completed
Type	Institute Project
Start date	04/10/2010
End date	03/10/2011
Duration	12 months

Abstract

Live attenuated and inactivated vaccines, produced in embryonated specific pathogen free hens eggs, are used to control infectious bronchitis virus (IBV). This is an expensive process both in handling terms and availability of eggs. Other vaccines, such as influenza virus, are produced in embryonated eggs but other safer and more efficient cell culture based systems are being used and developed. Recent work, resulting from a BBSRC DTA funded studentship, has shown that sequences in the S2 subunit allow the growth of IBV in Vero cells; an African green monkey cell line used for the production of vaccines. This project is to extend our initial proof of principle results that have shown we can produce rIBVs for potential vaccine purposes that can be grown in cell culture rather than in embryonated hens eggs. The work builds on our expertise on the manipulation of the IBV genome using reverse genetics and on our ability to produce recombinant viruses that express modified S proteins.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Animal Health, Immunology, Microbiology, Technology and Methods Development
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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