Award details

Correlation of immunogenicity with microarray analysis of vector mutants to improve live recombinant poxvirus vaccines in poultry

Reference	BBS/E/I/00001480
Principal Investigator / Supervisor	Dr Colin Butter
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	201,991
Status	Completed
Type	Institute Project
Start date	31/01/2012
End date	31/01/2016
Duration	48 months

Abstract

The project aims: (i) to identify differential changes in global gene expression in infected cells in vitro attributable to disruption of a wide range of non-essential genes of FWPV and (ii) to correlate those changes with differential immunogenicity of recombinant FWPV vectors carrying the genetic lesions.The mutants will be classified by the nature of the differential changes in global gene expression that they induce. Those that induce significant changes will be tested for differential induction of immune responses against foreign (AI) antigens in the permissive (avian) system. In this way the project aims to correlate differential changes in expression in the infected cells, attributable to the lack of expression of particular non-essential genes of FWPV, with differences in the efficacy of modified recombinant FWPV to stimulate immune responses against foreign antigens expressed by the recombinants. Ultimately, this should allow differential changes in global gene expression, induced in infected cells by variants of particular vectors in vitro, to be used as predictors of vaccine efficacy.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Animal Health, Immunology, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search