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BBSRC-Pfizer student: Analysis of an anti-silencing mechanism involved in immune evasion by vector borne dsRNA animal viruses of family Reoviridae

Reference	BBS/E/I/00001353
Principal Investigator / Supervisor	Dr Houssam Attoui
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	19,688
Status	Completed
Type	Institute Project
Start date	01/10/2007
End date	30/06/2012
Duration	57 months

Abstract

The reoviruses have 9 to 12 segmented dsRNA genomes and include important animal pathogens (including humans). The project will investigate the mechanisms these viruses use to evade RNA silencing. dsRNA-binding/helicase proteins from Bluetongue and Banna viruses, will be expressed to investigate their ability to inactivate silencing by sequestering/unwinding 21bp dsRNA silencing molecules. Antibodies to these proteins (e.g. Banna virus VP12 and orthoreovirus sigma 3) will be used to identify their sub-cellular location (confocal microscopy). Gel shift assays (using siRNAs) will be used to confirm dsRNA binding by the expressed proteins. Expression of native proteins in the cell will itself be subjected to silencing, by pre-treating cells with appropriate siRNAs. The same cells will then be transfected (TAT-mediated transfection) with anti-silencing proteins from other viruses (e.g. tombusvirus p19 protein), to elucidate the antisilencing mechanism involved

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Animal Health, Immunology, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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