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BBSRC-funded studentship: Identification of host factors which restrict African swine fever virus replication
Reference
BBS/E/I/00001349
Principal Investigator / Supervisor
Dr Linda Dixon
Co-Investigators /
Co-Supervisors
Institution
The Pirbright Institute
Department
The Pirbright Institute Department
Funding type
Research
Value (£)
23,300
Status
Completed
Type
Institute Project
Start date
01/10/2007
End date
30/09/2011
Duration
48 months
Abstract
The aims of the project are to understand at the molecular level the host factors which restrict efficient ASFV replication and to use this information to generate cell lines which are permissive for virus replication which could be used for growing vaccine strains. The hypothesis is that mature macrophages express cell surface and/or intracellular factors which are required for efficient replication of African swine fever virus (ASFV) and that cells non-permissive for infection do not express these. We will use microarrays to identify genes expressed in permissive compared to non-permissive cells. siRNAs will be used to knock down expression of these genes in permissive cells and the effect on virus replication analysed. Those genes identified as potentially required for replication will be stably transfected into non-permissive cell lines and virus replication tested. This general approach could be used for other pathogens.
Summary
unavailable
Committee
Not funded via Committee
Research Topics
Animal Health, Immunology, Microbiology
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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