Award details

Application of RT-PCR for the diagnosis of FMD and other vesicular diseases

Reference	BBS/E/I/00001198
Principal Investigator / Supervisor	Dr Donald King
Co-Investigators / Co-Supervisors	Dr Nigel Ferris, Professor David Paton
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	135,481
Status	Completed
Type	Institute Project
Start date	01/07/2005
End date	30/06/2008
Duration	36 months

Abstract

This project has the following objectives: (1) An inter-laboratory proficiency test involving national reference laboratories will be co-ordinated by IAH. This exercise will compare the performance of the Pirbright automated real-time RT-PCR assay with other molecular assays. Material suitable for use as standards for RT-PCR methods will be prepared and tested. (2) An encapsulated internal control to validate negative results will be developed for use in the TaqMan® RT-PCRs for FMDV and other vesicular disease viruses. (3) Multiplex assays will be developed that are capable of the simultaneous detection of a variety of PCR targets. These assays will be used for differential diagnosis of FMD from other vesicular disease viruses, and to provide serotype-specific and other characterisation data for FMDV isolates. (4) We will develop a modified single-tube RT-PCR that will be compatible with mobile PCR equipment that could be used in the field or located in regional laboratories. Since this technology is designed for use by non-laboratory personnel, these assays have the potential to rapidly deliver highly sensitive diagnostic results rapidly close to the suspect case of FMDV. (5) The suitability of alternative amplification and immunocapture template enrichment strategies will be investigated. These formats may be particularly suited for use in mobile/field-based situations (or in the developing world), where access to "fragile" precision equipment is not practical (or too expensive). Together, these objectives will aim to fully realise the potential of molecular methods such as RT-PCR for use in the detection of FMDV and other vesicular disease viruses.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	Animal Health, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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