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Consequences of foot and mouth disease virus infection on dendritic cell function

Reference	BBS/E/I/00001167
Principal Investigator / Supervisor	Professor Bryan Charleston
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	3,065
Status	Completed
Type	Institute Project
Start date	01/10/2004
End date	30/09/2007
Duration	36 months

Abstract

Dendritic cells (DCs) comprise the major antigen-presenting cells (APCs) of the host, uniquely programmed to stimulate immunologically naive T lymphocytes. Three different sources of cattle dendritic cells will be used for these studies, monocyte derived DC, dendritic cells harvested from skin and ALVC (afferent lymph veiled cells) harvested from pseudo-afferent lymph cannulation. Monocyte derived DCs and skin DCs will be isolated from porcine tissue. We propose to determine whether a range of different FMDV isolates are able to infect the different dendritic cell populations described above. Included in this range of viruses will be isolates that use the integrin avß6 exclusively for entry and isolates that have been passaged in vitro and have a broader tropism. Infected or control monocyte derived dendritic cells will be used to study the effects of FMDV infection on the capacity to stimulate either CD4 or CD8 T cell proliferation and cytokine production in response to specific antigen or as a consequence of allotypic stimulation. The opportunity may arise later in the project to develop viral vectors to target FMDV proteins to DCs, then study the T cell response stimulated by these different constructs.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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