Award details

Generation of molecular tools to study porcine lymphocytes

Reference	BBS/E/I/00001043
Principal Investigator / Supervisor	Dr Haru - Hisa Takamatsu
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	169,484
Status	Completed
Type	Institute Project
Start date	01/04/2003
End date	31/03/2006
Duration	36 months

Abstract

There is a need to generate new tools to study porcine lymphocyte populations. Traditionally, monoclonal antibodies would be raised against porcine lymphocytes and screened for those that show specificity for cells of immunological interest. The target antigen would then be characterised using biochemical methods. We will take a different approach in this project and exploit the growing information available in public databases to isolate porcine genes of immunological interest, and use these to generate antibodies from expressed protein. We have shown recently that porcine lymphocyte populations contain high levels of cells which resemble natural killer (NK) cells. Antibodies that recognise porcine NK receptors are not available at present, but sequences of the genes encoding the subunits of the porcine NKG2D/DAP receptor are available in the public database. This information will be used to isolate c-DNA¿s encoding these receptors for use in protein and antibody production. Molecular techniques will also be used to generate porcine MHC class 1 tetramers (see P0788). Again database sequences will be used to isolate c-DNAs encoding porcine MHC class 1 genes and these will be used to generate recombinant protein for tetramer construction.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search