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Genetic analysis of enterohaemorrhagic Escherichia coli colonisation and pathogenesis in cattle

Reference	BBS/E/I/00001013
Principal Investigator / Supervisor	Professor Mark Stevens
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	551,538
Status	Completed
Type	Institute Project
Start date	01/04/2003
End date	30/06/2009
Duration	75 months

Abstract

Analysis of the E. coli O157:H7 complete genome sequence has indicated that E. coli O157:H7 contains 1386 genes that are not present in the E. coli K-12 laboratory strain. Approximately 60% of these E. coli O157:H7-specific genes have no known function and their distribution in other EHEC serotypes in unknown. As part of an ongoing collaboration with Imperial College London Dr Stevens will use an oligonucleotide microarray based on the complete E. coli O157:H7 genome to study the prevalence of O157-specific genes in other EHEC serotypes (genome indexing) and to analyse the transcription of EHEC genes in vitro and in vivo. Studies have already been initiated to recover EHEC RNA from the intestines of gnotobiotic calves. If successful these studies will be extended to other EHEC serotypes that interact with the bovine intestine in a distinct way from E. coli O157:H7. Such studies will shed light on the timing and level of expression of EHEC genes during the interaction with the bovine host and will facilitate the development of intervention strategies.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	Animal Health, Microbial Food Safety, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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