Award details

Virulence Determinants in Rinderpest Virus

Reference	BBS/E/I/00001012
Principal Investigator / Supervisor	Professor Thomas Barrett
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	536,343
Status	Completed
Type	Institute Project
Start date	01/04/2003
End date	31/03/2008
Duration	60 months

Abstract

Revere genetics for RPV was developed some years ago in the Pirbright laboratory and is also the only way at present that virulence factors of RPV can be studied at the molecular level. In this project genomes from strains of different virulence will be sequenced in their entirety and assembled into full-length cDNAs for virus rescue. This approach has been successfully applied to identify attenuating mutations in the vaccine strain of RPV, which were found to be present in several of the structural protein genes and in the virus promoters. Variations that might explain pathogenic differences between field viruses can be identified in a similar way and these motifs/genes exchanged between the strains and their effects on virus pathogenicity determined experimentally in cattle. This will give an insight of how natural variation in the field affects virus virulence, which most probably will be different from the multiple mutations that have accumulated in the vaccine virus, and allow better predictions to be made on the likelihood of field reversion of mild to virulent forms.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search