Award details

Virulence Determinants in Rinderpest Virus

ReferenceBBS/E/I/00001012
Principal Investigator / Supervisor Professor Thomas Barrett
Co-Investigators /
Co-Supervisors
Institution The Pirbright Institute
DepartmentThe Pirbright Institute Department
Funding typeResearch
Value (£) 536,343
StatusCompleted
TypeInstitute Project
Start date 01/04/2003
End date 31/03/2008
Duration60 months

Abstract

Revere genetics for RPV was developed some years ago in the Pirbright laboratory and is also the only way at present that virulence factors of RPV can be studied at the molecular level. In this project genomes from strains of different virulence will be sequenced in their entirety and assembled into full-length cDNAs for virus rescue. This approach has been successfully applied to identify attenuating mutations in the vaccine strain of RPV, which were found to be present in several of the structural protein genes and in the virus promoters. Variations that might explain pathogenic differences between field viruses can be identified in a similar way and these motifs/genes exchanged between the strains and their effects on virus pathogenicity determined experimentally in cattle. This will give an insight of how natural variation in the field affects virus virulence, which most probably will be different from the multiple mutations that have accumulated in the vaccine virus, and allow better predictions to be made on the likelihood of field reversion of mild to virulent forms.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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