Award details

Structural and functional studies of bluetongue virus non-structural protein 2 (NS2(ViP)), and outer capsid proteins VP2 and VP5

Reference	BBS/E/I/00000950
Principal Investigator / Supervisor	Professor Peter Mertens
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	6,368
Status	Completed
Type	Institute Project
Start date	02/07/2002
End date	01/07/2005
Duration	36 months

Abstract

The bluetongue virus inclusion body matrix protein, NS2(ViP), is important in virus genome assembly and morphogenesis. Small crystals (0.05 mm long) of bacterial expressed NS2(ViP) generated weak X-ray diffraction data to beyond 4Å resolution. Crystallisation conditions and diffraction data will be improved to determine the protein¿s atomic structure. Difference imaging will identify NS2(ViP) binding sites for RNAs, nucleotides, metal ions etc. The role of NS2(ViP) in control of translation, packaging, helicase activity, sequestration of viral RNAs etc. will also be studied. Purified BTV infectious subviral particles (ISVP), in which outer capsid protein VP2 is cleaved, have produced fragile crystals, but as yet no useable diffraction data. The structure of the virus and ISVP outer capsid proteins will be studied by crystallography and cryo-electron microscopy.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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