Award details

The structure and function of the internal ribosome entry sites from foot and mouth disease and encephalomyocarditis virus RNAs

Reference	BBS/E/I/00000945
Principal Investigator / Supervisor	Professor Graham Belsham
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	7,721
Status	Completed
Type	Institute Project
Start date	01/05/2002
End date	30/04/2004
Duration	24 months

Abstract

The main objectives of the project are: 1. To obtain data on the three-dimensional organisation of the Internal Ribosomal Entry Sites (IRESs) of foot-and-mouth disease virus (FMDV) and encephalomyocarditis virus (EMCV) RNAs using a novel strategy of chemical crosslinking. 2. To determine a functional role for the central domain of FMDV and EMCV IRESs. This part of the work includes: a) assembly of the translation initiation complexes in vitro from individual components and determination of whether nucleotide residues of the apical part of the domain I are protected from chemical or RNAs attack in these complexes: b) identification of the translation initiation component(s) (if any) that result in protection of the nucleotides in the apical region of domain I of FMDV and EMCV IRESs; c) identification of the translation initiation component(s) that crosslink(s) to affinity labels attached to defined nucleotides of the apical region of domain I. 3. To use the translation directed in vitro by capped EMCV/FMDV IRES containing monocistronic mRNAs in HeLa cell extracts as a model to study a functional role of separate elements of the IRESs.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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