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Characterisation of bovine respiratory syncytial virus (BRSV)-specific bovine CD8+ T cells

Reference	BBS/E/I/00000938
Principal Investigator / Supervisor	Dr Geraldine Taylor
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	29,801
Status	Completed
Type	Institute Project
Start date	21/10/2002
End date	31/03/2007
Duration	53 months

Abstract

Bovine respiratory syncytial virus (BRSV) is a major cause of pneumonia in young calves and is also a useful model of the closely related human (H)RSV. CD8+ T cells play a crucial role in recovery from RSV infections and factors that influence CTL priming or the induction of CTL memory may influence the outcome of RSV infection. Thus, poor CTL memory may be responsible for the observed frequency of RSV reinfection. Identification of the antigenic specificity of BRSV-specific CD8+ T cells will allow a detailed quantitative and qualitative analysis of the CD8+ T cell response in calves; provide insight into the relationship between BRSV-specific CD8+ T cell memory and frequency of reinfection; and enable investigation of factors that regulate the generation and long-term survival of BRSV-specific CD8+ T cells. The aims of this work are therefore to (1) determine the influence of bovine MHC class I genotype on the BRSV proteins and epitopes recognised by BRSV-specific bovine CD8+ T cells and (2) to determine the role of BRSV proteins on the generation and long-term survival of CD8+ T cell memory in calves infected with wild-type BRSV or recombinant (r)BRSV. The proteins and epitopes recognised by BRSV-specific CD8+ T cells from MHC-defined calves will be identified using recombinant vaccinia and fowlpox viruses expressing individual BRSV proteins and using synthetic peptides in Elispot assays to analyse IFN? production by CD8 T cells.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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