Award details

Cattle T cell responses and dendritic cell sub-populations

Reference	BBS/E/I/00000928
Principal Investigator / Supervisor	Professor Christopher Howard
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	572,697
Status	Completed
Type	Institute Project
Start date	01/04/2002
End date	31/03/2005
Duration	36 months

Abstract

Dendritic cells are the only antigen presenting cell considered capable of initiating primary T cell responses in naïve animals. In cattle ¿ex-vivo¿ dendritic cells can be isolated from the afferent lymphatics draining the skin. A number of sub-populations of these dendritic cells are evident, as defined by staining with monoclonal antibodies. Ex-vivo cells will be compared with dendritic cells derived from precursors in blood by culture with cytokines that provide a model for less mature cells. Differences in the synthesis of cytokines and co-stimulatory surface molecules by dendritic cell sub-populations that will influence the bias of the T cell response are evident. Synthesis of cytokine transcripts and protein by the dendritic cell sub-populations and expression of established and novel co-stimulatory surface molecules by dendritic cell sub-populations will be determined. The response of subsets of bovine CD4, CD8 and gamma delta TCR+ T cells to antigens presented by the dendritic cells and the effect on the bias and type of the T cell response will be assessed. This will enable the manner in which the dendritic cells and T cells interact and respond to bacterial, viral and model antigens and how this has the potential to influence resistance to disease to be examined.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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