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Analysis of gene 5 of the avian coronavirus infectious bronchitis virus utilising a reverse genetics system

Reference	BBS/E/I/00000882
Principal Investigator / Supervisor	Professor David Cavanagh
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	130,970
Status	Completed
Type	Institute Project
Start date	01/10/2001
End date	30/09/2004
Duration	36 months

Abstract

Avian infectious bronchitis virus (IBV) is a coronavirus, having a 27.6 kb single-stranded, positive sense RNA genome. In addition to gene 1, which encodes the replicase, there are two genes that encode non-structural proteins. One of these, gene 5, has two open reading frames (ORFs), encoding proteins 5a and 5b. They are produced in IBV-infected cells but nothing is known about there function. As a first step in the elucidation of the roles of these proteins, we are preparing knock-out mutants, both of each ORF and of the upstream transcription-associated sequences, so as to also prevent gene 5 mRNA production. Recombinant IBVs containing these mutations will be produced using our recently developed reverse genetics system, after which the replication properties of the recombinants will be studied. Monospecific antisera are being prepared against 5a and 5b proteins, to identify their cellular location and to ascertain if they interact with other proteins, both viral and cellular.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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