Award details

Defining the repertoire of the microneme and rhoptry organelles of the apicomplexan parasite Eimeria tenella by cell map proteomics

Reference	BBS/E/I/00000864
Principal Investigator / Supervisor	Professor Fiona Tomley
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	38,999
Status	Completed
Type	Institute Project
Start date	03/09/2001
End date	02/09/2004
Duration	36 months

Abstract

The objectives of the project are: 1. To produce two dimensional protein maps for micronemes and rhoptries of E.tenella sporozoites 2. To use peptide mass spectrometry to produce peptide map profiles and limited amino acid sequences for microneme and rhoptry proteins 3. To identify genes enclosing these proteins by comparing peptide profiles and amino acid sequences with computer-generated peptide profiles and open reading frames from E.tenella ESTs and genomic sequences 4. To incorporate these data into a publicly available core E.tenella database 5. To sequence full-length cDNAs of selected proteins and verify their subcellular localisation in the parasite with antibodies raised against expressed recombinant proteins

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search