Award details

Genetic recombination of traits for protective immunity and drug-resistance in the protozoan Eimeria maxima from the fowl

Reference	BBS/E/I/00000863
Principal Investigator / Supervisor	Professor Martin Shirley
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	40,120
Status	Completed
Type	Institute Project
Start date	01/10/2001
End date	30/09/2003
Duration	24 months

Abstract

The overall objective of the work is to establish that for Eimeria maxima genetic loci encoding the phenotypes of antigenic type segregate independently of resistance to an anticoccidial drug (robenidine). This is the first part of a new strategy that aims to identify the antigens in an antigenically complex protozoan parasite that induce protective immunity during the course of natural infection. As we expect this first part of the work to be successful, we will seek further funding to map and positionally clone the genetic loci responsible. This work divides into: 1. Derivation of a series of genetic crosses between two antigenically distinct populations of E.maxima, defined also by drug-resistance or drug-sensitivity. 2. Selection of progeny in the presence of both selectable markers and confirmation by in vivo and in vitro tests that the parasites are characterised by unique recombinant phenotypes of antigenic type and drug-resistance. The successful outcome of the work will allow the derivation of clonal and non-clonal populations of recombinant parasites that can be analysed for polymorphic DNA markers to identify genetic loci that encode protective antigens. These protective antigens will provide the foundation for the development of an effective recombinant vaccine against coccidiosis.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search