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Molecular basis of salmonella virulence

Reference	BBS/E/I/00000858
Principal Investigator / Supervisor	Dr Edouard Galyov
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	260,254
Status	Completed
Type	Institute Project
Start date	01/04/2001
End date	31/03/2004
Duration	36 months

Abstract

Our objective is to understand how different Salmonella Sop and Pip effector proteins function inside host cells to elicit proinflammatory responses that lead to intestinal inflammation. We speculate that some Sops and Pips may be needed for the induction of signals leading to the attraction of PMN from the peripheral blood into the lamina propria, whereas, others may be involved in orchestrating the migration of PMNs across the epithelial monolayers. We will construct and use genetically defined Salmonella mutants in association with our biologically relevant model systems to examine the importance of effector proteins in enteropathogenicity. To enable further functional analyses, we will assemble eukaryotic expression plasmids encoding Sop and Pip effector proteins and assess effects mediated by their expression in cultured cells. We will localize effector proteins to specific cellular organelles and analyze the targeting signals and subsequent cellular responses. The rationale for this is based on the knowledge that different effector proteins (Yops) of Yersinia are targeted to different cellular compartments. Finally, we will assess the relevance of the data obtained in vitro by performing experimental Salmonella infections using the relevant animal model system and monitoring the responses elicited by such infections.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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