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Control of chicken class I peptide-binding specificities by polymorphic chicken TAPS

Reference	BBS/E/I/00000842
Principal Investigator / Supervisor	Professor Jim Kaufman
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	98,556
Status	Completed
Type	Institute Project
Start date	21/06/2001
End date	04/06/2004
Duration	35 months

Abstract

This work aims to test the hypothesis that in chickens, in contrast to well-studied mammals, the specificity of the immune response to class I molecules is determined strongly by the specificities of the transporters associated with antigen presentation (TAPs). There are three broad objectives, each using three chicken MHC haplotypes (B4, B15 and B21). In vitro assays will be used to compare the peptide-translocation specificities of TAPs with the peptide-binding specificities of class I molecules and with the peptide motifs previously determined for class I molecules isolated from cells. Normal and transfected cells will be used to examine the specificity of interactions of peptides, TAPs and class I molecules in vivo, in particular to examine the relative contributions of peptide availability versus TAP/class I binding in determining the repertoire of peptides presented as well as the cell surface expression level of class I molecules. In vitro translocation assays will be used to approach the potential role of TAPs in viral immune evasion, beginning with Marek's disease virus. Peptide-translocation will be analysed in the first year and peptide-binding in the second year. The transfection system will be set up in the first year and used to test all combinations over the following 18 months. Heterozygote normal cells will be analysed in the second year and the effects of viral lysates on translocation will be analysed in the final year.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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