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Probing conformational changes in the structure of prion proteins using nucleic acid ligands

Reference	BBS/E/I/00000834
Principal Investigator / Supervisor	Professor Jim Kaufman
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	11,960
Status	Completed
Type	Institute Project
Start date	02/10/2000
End date	01/10/2003
Duration	36 months

Abstract

Probing conformational changes in the structure of prion proteins using nucleic acid ligands. RNA ligands for the prion protein have been developed in collaboration with Oxford University and the student is investigating the interactions of these molecules with wild-type and truncated/mutated forms of purified recombinant prion protein by gel shift analysis, analytical ultracentrifugation and various spectroscopic methods. The objective is to obtain high resolution structural data on the prion:RNA complex, to understand at the atomic level the basis of the specificity of this interaction and to use this information to guide a structure-based molecular design approach for better ligands. Such ligands have potential for diagnostic and therapeutic applications in the TSE field.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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