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Award details
Organisation of the I megabase (smallest) chromosome from the poultry coccidium Eimeria tenella
Reference
BBS/E/I/00000833
Principal Investigator / Supervisor
Professor Martin Shirley
Co-Investigators /
Co-Supervisors
Institution
The Pirbright Institute
Department
The Pirbright Institute Department
Funding type
Research
Value (£)
11,700
Status
Completed
Type
Institute Project
Start date
02/10/2000
End date
01/10/2003
Duration
36 months
Abstract
The primary objective of this project is to determine the physical organisation of chromosome 1 of E. tenella and to integrate the data with the existing genetic linkage map. Specifically, the student is likely to (1) assemble overlapping cosmid clones using the technique of thanchromosome walkingsuch to form a contigious linear map (2) place on the map chromosome- specific anonymous markers derived from the genetic linkage mapping work, EST and gene markers (3) focus more closely on the region of chromosome 1 that is implicated in resistance to arprinocid - a study that is likely to include DNA sequence analyses (4) attempt to identify the region(s) and the molecular basis of the size polymorphisms in the chromosome that characterise different strains of the parasite. The student will receive training in a wide range of techniques in molecular biology, including working with cosmids, PCR methods, DNA cloning and sequencing, hybridisation assays, data analyses using a range of computer software, etc. In addition, a broad practical understanding in the field of parasitology will be provided together with guidance for the written and oral presentation of results.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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