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Post-genomic studies of fowlpox virus replication and host interactions

Reference	BBS/E/I/00000784
Principal Investigator / Supervisor	Professor Michael Skinner
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	335,280
Status	Completed
Type	Institute Project
Start date	01/04/2000
End date	31/03/2003
Duration	36 months

Abstract

Objectives: 1) To investigate the specificity of candidate immunomodulatory proteins of FP9. The genes encoding relevant targets and competitors (receptors and ligands) from chickens (the natural host) are just becoming available from avian genetics & immunology programmes at IAH and elsewhere. Because of the interest in use of FPV as a non-replicating vector in animals and Man, we are also interested in determining whether the FPV immunomodulators have any coincidental cross-reactivity with targets from those non-permissive, mammalian hosts. Any such interaction could have subtle, but potentially important, implications for the efficacy of vaccination by non-replicating recombinant vectors. 2) To investigate interactions between FPV proteins and between FPV and host proteins using a proteomics approach. Although we have isolated a number of monoclonals against FPV structural proteins and, using them, have made some progress on FPV morphogenesis, we wish to use modern approaches to speed the analysis of morphogenesis (which appears to be involved in FPV host-range restriction). The interactions between FPV proteins will be a genome wide yeast 2-hybrid study of the type used recently for yeast and for vaccinia virus.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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