Award details

Chicken immunity and resistance to disease based upon analysis of gene expression (Chicken Image)

Reference	BBS/E/I/00000768
Principal Investigator / Supervisor	Dr N Bumstead
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	228,660
Status	Completed
Type	Institute Project
Start date	01/02/2000
End date	01/09/2003
Duration	43 months

Abstract

The objectives of this project are: 1. To create an immunologically relevant genomic resources for the chicken (delivered by workpackage 1), made up of clusters of immunologically important genes, based on a resource of BAC end sequences (checked by milestone M1 and will give deliverables d1, d2) and delineating the repertoire of gene transcripts within these clusters (deliverable d3). 2. To construct a multi-tissue normalised cDNA library enriched in full-length clones (delivered by workpackage 2), representing the majority of chicken transcripts in immune tissues with active respones (deliverable d4). 3. To characterise a set of 30,000 cDNA clones from the normalised library by sequencing their ends, half of which should represent unique gene transcripts (workpackage 2, deliverable d5). To generate a specialised miniature cDNA array of non redundnat cDNA clones for up to 15,000 immune gene transcripts (delivered by workpackage 3, checked by milestone M3, M4) and use it to monitor the level of expression and tissue specificity of the corresponding genes (milestone M5, deliverables d6, d7). To identify candidate genes involved in resistance to Marek's disese, coccidiosis, and infectious bursal disease, which are among the principal pathogens of poultry in Europe, by exploring transcription profiles using well defined biological samples derived from resistant and susceptible birds (workpackage 4, deliverable d8, d9, d10) To establish genotyping and expression profile-based tests for field testing by the poultry industry (workpackage 5, deliverables d11, d12).

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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