Award details

Reverse genetics of respiratory syncytial virus: generation of attenuated vaccine candidates

Reference	BBS/E/I/00000767
Principal Investigator / Supervisor	Dr Geraldine Taylor
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	241,220
Status	Completed
Type	Institute Project
Start date	01/02/2000
End date	31/01/2002
Duration	24 months

Abstract

This project is focussed on obtaining vaccine candidates for both human and bovine respiratory syncytial viruses, major causes of severe lower respiratory tract infections in very young children and calves, respectively. Reverse genetic techniques, recently developed in the participating laboratories, will be used for in vitro manipulation of plasmids carrying the entire sequence of the viral genomes in cDNA forms. Viruses will be rescued from mutagenised plasmids upon transfection of susceptible cells in tissue culture. In vitro assays will be developed to test the attenuated growth of mutant viruses and their capacity to induce the expression of key regulatory molecules of the inflammatory response. Finally, the immunological and pathological potential of the vaccine candidates will be evaluated in experimental animal models.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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