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Characterisation of the interaction of enterohaemorrhagic E. coli with intestinal mucosae
Reference
BBS/E/I/00000754
Principal Investigator / Supervisor
Dr Timothy Wallis
Co-Investigators /
Co-Supervisors
Institution
The Pirbright Institute
Department
The Pirbright Institute Department
Funding type
Research
Value (£)
143,370
Status
Completed
Type
Institute Project
Start date
01/02/1999
End date
31/01/2002
Duration
36 months
Abstract
Enterohaemorrhagic E. coli [EHEC] infections are an increasingly important zoonosis. Putative virulence factors have been identified, but their role in pathogenesis and host specificity remains unclear. We aim to study the interaction of wild type and defined mutant strains of EHEC with intestinal mucosae from humans and cattle, natural host species. Early host/pathogen interactions will be assessed in primary intestinal biopsies using in vitro organ culture. Subsequent events in pathogenesis will be studied using the ligated intestinal loop model in cattle which allows quantification of intestinal invasion, secretory and inflammatory responses. Translocation of specific virulence factors will be assessed by immunofluorescent and confocal microscopy. Signature tagged mutagenesis will be exploited in vivo to identify novel virulence genes.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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