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Genes of Salmonella typhimurium involved in in vitro and in vivo stationery-phase growth

Reference	BBS/E/I/00000753
Principal Investigator / Supervisor	Professor Paul Barrow
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	53,600
Status	Completed
Type	Institute Project
Start date	01/10/1998
End date	30/09/2001
Duration	36 months

Abstract

When newly hatched chickens are inoculated orally with a Salmonella strain the bacteria multiply extensively to high numbers and confer resistance to reinfection as a microbiological phenomenon. The practical value of this is that live attenuated Salmonella vaccines may be administered orally to newly hatched chicks which are then protected within 24 hours by this exclusion effect. Salmonella genes that are expressed in the alimentary tract of very young chickens are different to those required for survival in the gut of adult birds. In young birds the microbial growth characteristics are similar to those seen in stationary phase nutrient broth cultures and this may be used as a model system. It is known that genes responsible for stationary phase gene regulation (rpoS) and cell division (sdiA) are expressed at different stages in early stationary phase. We are seeking to look at the effect on their expression of introducing other mutations into S. typhimurium. Fusions have been constructed between these genes and luxAB and mutations involving the electron transport chain, which affect stationary phase growth have been introduced.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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