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Interactions between African swine fever virus encoded proteins and host proteins

ReferenceBBS/E/I/00000747
Principal Investigator / Supervisor Dr Linda Dixon
Co-Investigators /
Co-Supervisors
Institution The Pirbright Institute
DepartmentThe Pirbright Institute Department
Funding typeResearch
Value (£) 134,884
StatusCompleted
TypeInstitute Project
Start date 01/04/2000
End date 31/03/2003
Duration36 months

Abstract

The objectives are to identify host pig macrophage proteins that bind to ASFV encoded proteins in order to predict functions for the virus encoded proteins. This will be achieved by screening a pig macrophage cDNA library with 7-10 different ASFV genes using the yeast two-hybrid screen. Sequences of inserts in clones encoding interacting proteins will be determined and the interactions confirmed by in vitro binding, co-precipitation from cell extracts and co-localisation in cells. Predicted functions for the virus encoded proteins will then be tested using methodology appropriate for that function.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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